|Title||Allopregnanolone reverses neurogenic and cognitive deficits in mouse model of Alzheimer's disease.|
|Publication Type||Journal Article|
|Year of Publication||2010|
|Authors||Wang JMing, Singh C, Liu L, Irwin RW, Chen S, Chung EJi, Thompson RF, Brinton RDiaz|
|Journal||Proc Natl Acad Sci U S A|
|Date Published||2010 Apr 06|
|Keywords||Alzheimer Disease, Amyloid beta-Peptides, Animals, Cell Proliferation, Disease Models, Animal, Humans, Learning, Male, Memory, Mice, Mice, Transgenic, Neurogenesis, Neurons, Pregnanolone, Stem Cells|
Our previous analyses showed that allopregnanolone (APalpha) significantly increased proliferation of rodent and human neural progenitor cells in vitro. In this study, we investigated the efficacy of APalpha to promote neurogenesis in the hippocampal subgranular zone (SGZ), to reverse learning and memory deficits in 3-month-old male triple transgenic mouse model of Alzheimer's (3xTgAD) and the correlation between APalpha-induced neural progenitor cell survival and memory function in 3xTgAD mice. Neural progenitor cell proliferation was determined by unbiased stereological analysis of BrdU incorporation and survival determined by FACS for BrdU+ cells. Learning and memory function was assessed using the hippocampal-dependent trace eye-blink conditioning paradigm. At 3 months, basal level of BrdU+ cells in the SGZ of 3xTgAD mice was significantly lower relative to non-Tg mice, despite the lack of evident AD pathology. APalpha significantly increased, in a dose-dependent manner, BrdU+ cells in SGZ in 3xTgAD mice and restored SGZ proliferation to normal magnitude. As with the deficit in proliferation, 3xTgAD mice exhibited deficits in learning and memory. APalpha reversed the cognitive deficits to restore learning and memory performance to the level of normal non-Tg mice. In 3xTgAD mice, APalpha-induced survival of neural progenitors was significantly correlated with APalpha-induced memory performance. These findings suggest that early neurogenic deficits, which were evident before immunodetectable Abeta, may contribute to the cognitive phenotype of AD, and that APalpha could serve as a regenerative therapeutic to prevent or delay neurogenic and cognitive deficits associated with mild cognitive impairment and Alzheimer's disease.
|Alternate Journal||Proc. Natl. Acad. Sci. U.S.A.|
|PubMed Central ID||PMC2851948|
|Grant List||U01 AG031115 / AG / NIA NIH HHS / United States |
P01 AG02657 / AG / NIA NIH HHS / United States
U01AG031115 / AG / NIA NIH HHS / United States
Allopregnanolone reverses neurogenic and cognitive deficits in mouse model of Alzheimer's disease.
Faculty Member Reference:
Roberta Diaz Brinton, Ph.D