Laminin-5-mediated gene expression in human prostate carcinoma cells.

TitleLaminin-5-mediated gene expression in human prostate carcinoma cells.
Publication TypeJournal Article
Year of Publication2001
AuthorsCalaluce R, Kunkel MW, Watts GS, Schmelz M, Hao J, Barrera J, Gleason-Guzman M, Isett R, Fitchmun M, Bowden GT, Cress AE, Futscher BW, Nagle RB
JournalMol Carcinog
Date Published2001 Feb
KeywordsBlotting, Northern, Cell Adhesion Molecules, DNA, Complementary, DNA, Neoplasm, Gene Expression, Gene Expression Profiling, Humans, Immunoenzyme Techniques, Male, Neoplasm Proteins, Oligonucleotide Array Sequence Analysis, Prostatic Neoplasms, Tumor Cells, Cultured

Interactions between extracellular matrix (ECM) proteins and prostate carcinoma cells provide a dynamic model of prostate tumor progression. Previous work in our laboratory showed that laminin-5, an important member of a family of ECM glycoproteins expressed in the basal lamina, is lost in prostate carcinoma. Moreover, we showed that the receptor for laminin-5, the alpha6beta4 integrin, is altered in prostate tumors. However, the genes that laminin-5 potentially regulates and the significance of its loss of expression in prostate cancer are not known. We selected cDNA microarray as a comprehensive and systematic method for surveying and examining gene expression induced by laminin-5. To establish a definitive role for laminin-5 in prostate tumor progression and understand the significance of its loss of expression, we used a cDNA microarray containing 5289 human genes to detect perturbations of gene expression when DU145 prostate carcinoma cells interacted with purified laminin-5 after 0.5, 6, and 24 h. Triplicate experiments showed modulations of four, 61, and 14 genes at 0.5, 6, and 24 h, respectively. Genes associated with signal transduction, cell adhesion, the cell cycle, and cell structure were identified and validated by northern blot analysis. Protein expression was further assessed by immunohistochemistry. Mol. Carcinog. 30:119-129, 2001.

Alternate JournalMol. Carcinog.
PubMed ID11241759
Grant ListP30 CA023074 / CA / NCI NIH HHS / United States
CA78447 / CA / NCI NIH HHS / United States
P01 CA56666-05 / CA / NCI NIH HHS / United States